Back-​Scattering Interferometry: An Ultrasensitive Method for the Unperturbed Detection of Acetylcholinesterase-​Inhibitor Interactions

TitleBack-​Scattering Interferometry: An Ultrasensitive Method for the Unperturbed Detection of Acetylcholinesterase-​Inhibitor Interactions
Publication TypeJournal Article
Year of Publication2012
AuthorsHaddad GL
Secondary AuthorsYoung SC, Heindel ND, Bornhop DJ, Flowers, II RA
JournalAngewandte Chemie, International Edition
Volume51
Issue44
Start Page11126
Pagination4
Abstract

Back-​Scattering Interferometry (BSI) provides a highly sensitive method for the detection of anticholinesterases with a range of potencies and inhibition types. Unlike other methods, BSI does not require the use of substrate (or a deriv. thereof) for signal generation, thus avoiding the possibility of false signals. Using the multipass configuration of BSI, a detection limit of 3.6 x IO-​5 fmol of AChE was obtained for an optical probe vol. of 360 pL, a sensitivity which surpasses that of previously described colorimetric, fluorescent, chemiluminescent, and electrochem. techniques. BSI has the potential to track events other than target binding, such as metal complexation, liposome formation, and intermol. interactions of medically and biol. relevant mols.